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宿主来源
Rabbit抗原名称
NeuroD2分子别名
Neurogenic differentiation factor 2; Class A basic helix-loop-helix protein 1 (bHLHa1); NeuroD-related factor (NDRF); BHLHA1; NDRF; NEUROD2免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q15784克隆号
S-3092-64抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
mouse cerebellum, mouse brain, rat cerebellum, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:500-1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms IF 1:500 Ms
NeuroD2 is a neuron-specific basic helix-loop-helix transcription factor that drives cortical projection neuron differentiation, radial migration and layer-specific identity by directly activating genes like Cdk5r1, Lrp8, Fezf2 and Satb2, while it fine-tunes store-operated Ca²⁺ entry by repressing STIM1, and its deletion disrupts thalamocortical barrel map formation, reduces excitability and produces autism-like motor and seizure phenotypes.
免疫印迹
WB result of NeuroD2 Recombinant Rabbit mAb
Primary antibody: NeuroD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 48 kDa
This blot was developed with high sensitivity substrateWB result of NeuroD2 Recombinant Rabbit mAb
Primary antibody: NeuroD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Lane 3: rat brain lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 48 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human hippocampus. Anti-NeuroD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse hippocampus. Anti-NeuroD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NeuroD2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Negative control: IF shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-NeuroD2 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NeuroD2 in tumor tissue.
Expression of NeuroD2 in human tissue.
Expression of NeuroD2 in mouse & rat tissue.
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