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Rabbit抗原名称
MARCKS分子别名
Myristoylated alanine-rich C-kinase substrate; Protein kinase C substrate; 80 kDa protein; light chain (80K-L protein; PKCSL); MACS; PRKCSL免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cytoskeleton, Cell membraneAccession
P29966克隆号
S-3190-72抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, HEK-293纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu ICC 1:500 Hu
MARCKS (Myristoylated Alanine-Rich C Kinase Substrate) is an evolutionarily conserved, intrinsically disordered peripheral membrane protein that serves as a key phosphorylation substrate for conventional and novel protein kinase C isoforms; by reversibly sequestering phosphatidylinositol-4,5-bisphosphate (PIP2) and cross-linking actin filaments, it integrates PKC, Ca2+/calmodulin, and small GTPase signaling to orchestrate dynamic membrane-cytoskeleton remodeling underlying cell adhesion, migration, endo/exocytosis, neurotransmitter release, and stem-cell differentiation, while its dysregulation contributes to cancer metastasis, inflammatory diseases, and neuronal disorders.
免疫印迹
WB result of MARCKS Recombinant Rabbit mAb
Primary antibody: MARCKS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 80 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-MARCKS antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A431 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-MARCKS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of MARCKS in tumor tissue.
Expression of MARCKS in human tissue.
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