分享产品介绍 评论(0)
宿主来源
Rabbit抗原名称
KCC2分子别名
Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2 (hKCC2); Neuronal K-Cl cotransporter; KIAA1176; SLC12A5免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9H2X9克隆号
S-3044-2抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
unboiled mouse brain, unboiled rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt IHC-P 1:1000 Hu, Ms, Rt IF 1:500 Ms, Rt
KCC2 (potassium-chloride cotransporter 2) is a neuron-specific chloride-extruding transporter encoded by the SLC12A5 gene that maintains low intracellular Cl⁻ by coupling K⁺ and Cl⁻ efflux, thereby setting the hyperpolarizing, inhibitory polarity of GABA_A and glycine receptor currents essential for central nervous system inhibition, synapse maturation, dendritic spine formation, and neuronal survival, while its transcriptional and post-translational regulation (phosphorylation, membrane trafficking, activity-dependent expression) drives the developmental GABA “switch” from depolarizing to hyperpolarizing, and its down-regulation or dysfunction—triggered by epilepsy, trauma, inflammation, or genetic mutations—produces chloride accumulation, excitatory GABA, network hyperexcitability, and contributes to epilepsies, chronic pain, autism, and neurodegeneration, making KCC2 a genetically validated therapeutic target whose potentiation can restore inhibition and rescue drug-resistant neurological disorders.
免疫印迹
WB result of KCC2 Recombinant Rabbit mAb
Primary antibody: KCC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 126 kDa
Observed MW: 180 kDaWB result of KCC2 Recombinant Rabbit mAb
Primary antibody: KCC2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 126 kDa
Observed MW: 180 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCC2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-KCC2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti-KCC2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of KCC2 in tumor tissue.
Expression of KCC2 in human tissue.
Expression of KCC2 in mouse & rat tissue.
评论(0)