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宿主来源
Rabbit抗原名称
Dicer分子别名
Endoribonuclease Dicer; Helicase with RNase motif (Helicase MOI); DICER; HERNA; KIAA0928; DICER1免疫原
Recombinant Protein细胞定位
CytoplasmAccession
Q9UPY3克隆号
S-2497-202抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv, Ck, Hm, Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
Dicer is a multidomain RNase III enzyme that recognizes double-stranded RNA (dsRNA) or short-hairpin RNA (shRNA), measures ~22 nt from the 3′ overhang, and cleaves both strands to generate small interfering RNAs or microRNAs bearing 2-nt 3′ overhangs; these products are then loaded into Argonaute to form the RNA-induced silencing complex that drives gene silencing, making Dicer the central processor of the RNA interference pathway essential for development, viral defense, and genome stability.
免疫印迹
WB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Negative control: U937 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 219 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrateWB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 219 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrateWB result of Dicer Recombinant Rabbit mAb
Primary antibody: Dicer Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat testis lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 220 kDa
Observed MW: 240 kDa
This blot was developed with high sensitivity substrate
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