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宿主来源
Rabbit抗原名称
Collagen II分子别名
Collagen alpha-1(II) chain; Alpha-1 type II collagen; COL2A1细胞定位
SecretedAccession
P02458克隆号
S-3360抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:200 Hu, Rt
Collagen II, also known as type II collagen, is a crucial fibrillar protein predominantly found in cartilaginous tissues, including hyaline cartilage and articular cartilage, where it constitutes up to 90% of the collagen content. It is synthesized by chondrocytes and is essential for maintaining the structural integrity and resilience of cartilage. Collagen II is formed by homotrimers of the collagen type II alpha-1 chain (α1(II)), and its molecular structure includes a central triple-helical domain flanked by N- and C-propeptides. This collagen is also present in the vitreous humor of the eye, intervertebral discs, and the inner ear. Mutations in the gene encoding Collagen II (COL2A1) are associated with various skeletal and connective tissue disorders, such as Stickler syndrome and Kniest dysplasia.
免疫组化
IHC shows positive staining in paraffin-embedded rat cartilage. Anti-Collagen II antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat cartilage. Anti-Collagen II antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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