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COL1A1 Recombinant Rabbit mAb (S-2975-9)

Collagen alpha-1(I) chain,Alpha-1 type I collagen,Cola1,Col1a1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6538
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    COL1A1

  • 分子别名

    Collagen alpha-1(I) chain; Alpha-1 type I collagen; Cola1; Col1a1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Secreted

  • Accession

    P11087

  • 克隆号

    S-2975-9

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:5000 Hu, Ms
    IHC-P 1:2000 Hu, Ms, Rt
    ICC 1:500 Hu
    IF 1:100 Ms, Rt
背景介绍

  • COL1A1 (collagen type I alpha 1 chain) is the ~1,464-amino-acid pro-alpha1 strand that, together with two COL1A2 pro-alpha2 strands, assembles the triple-helical type I collagen—the main load-bearing protein of bone, skin, tendon, dentin and most connective tissues; after secretion, its N- and C-propeptides are cleaved by BMP-1/tolloid proteases, allowing the rigid 300-nm collagen fibrils to form and then be mineralized (in bone) or cross-linked (in soft tissues), while intracellularly it requires proline and lysine hydroxylation, endoplasmic-reticulum chaperones and trimer registration to prevent misfolding; mutations cause osteogenesis imperfecta, Ehlers–Danlos syndrome, Caffey disease and fibrosis, and its expression is driven by the Sp1/3, TGF-β/Smad and HIF pathways, is up-regulated in fibrosis and cancer stroma, and serves as a biomarker for tissue repair and invasiveness.

  • 免疫印迹

    • WB result of COL1A1 Recombinant Rabbit mAb
      Primary antibody: COL1A1 Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: HT-29 whole cell lysate 20 µg
      Lane 2: HFF whole cell lysate 20 µg
      Negative control: HT-29 whole cell lysate
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 138 kDa
      Observed MW: 220 kDa

    • WB result of COL1A1 Recombinant Rabbit mAb
      Primary antibody: COL1A1 Recombinant Rabbit mAb at 1/5000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 138 kDa
      Observed MW: 220 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-COL1A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-COL1A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-COL1A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse stomach. Anti-COL1A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti-COL1A1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HFF cells (top panel) and negative staining in HT-29 cells (below panel). Anti-COL1A1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse skin. Anti-COL1A1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded rat skin. Anti-COL1A1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 组织表达图谱

    • Expression of COL1A1 in tumor tissue.

    • Expression of COL1A1 in human tissue.

    • Expression of COL1A1 in mouse & rat tissue.

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