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宿主来源
Rabbit抗原名称
ALPG分子别名
Alkaline phosphatase, germ cell type; ALP-1; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase; placental-like; Germ cell alkaline phosphatase (GCAP); Placental alkaline phosphatase-like (PLAP-like); ALPPL; ALPPL2免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P10696抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
A431, PANC-1纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu IHC-P 1:1000 Hu
ALPG, also known as ALPPL2 or germ-cell alkaline phosphatase, is a 532-amino-acid, GPI-anchored glycoprotein enzyme (EC 3.1.3.1) belonging to the alkaline phosphatase family that is normally restricted to testis, thymus and trophoblast but becomes aberrantly over-expressed on the surface of a broad spectrum of solid tumours—including seminoma, mesothelioma, ovarian, pancreatic and gastric cancers—where it catalyzes the hydrolysis of phosphate monoesters, contributes to tumour growth and invasion, and serves as both a cancer-specific therapeutic target and a cell-surface marker for naïve pluripotency in stem-cell biology.
免疫印迹
WB result of ALPG Rabbit pAb
Primary antibody: ALPG Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: PANC-1 whole cell lysate 20 µg
Low expression control: HeLa whole cell lysate; A549 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 65 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human placenta. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human seminoma. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-ALPG antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ALPG in tumor tissue.
Expression of ALPG in human tissue.
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