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Rabbit抗原名称
Beta I tubulin分子别名
Tubulin beta-1 chain; TUBB1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q9H4B7克隆号
S-3108-63抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
A204, HepG2, HT-29, HeLa, NIH/3T3, PC-12, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu, Ms, Rt, Mk IHC-P 1:4000 Hu, Ms, Rt ICC 1:500 Hu, Ms
Beta I tubulin, the most neuron-enriched β-tubulin isoform encoded by TUBB1, is a ~50 kDa globular protein that heterodimerizes with α-tubulin to assemble neuron-specific microtubule arrays, stabilizing axonal and dendritic cytoskeletons through GTP-dependent head-to-tail polymerization and interaction with microtubule-associated proteins; its unique C-terminal tail undergoes reversible glutamylation and detyrosination to regulate motor-protein trafficking, synaptic plasticity and axonal transport, while missense mutations (e.g., V180A, R215W) disrupt microtubule dynamics and cause a spectrum of neurodevelopmental disorders termed “tubulinopathies” characterized by simplified gyral pattern, microcephaly and intellectual disability, making Beta I tubulin a critical determinant of neuronal morphology and human brain development.
免疫印迹
WB result of Beta I tubulin Recombinant Rabbit mAb
Primary antibody: Beta I tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A204 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDaWB result of Beta I tubulin Recombinant Rabbit mAb
Primary antibody: Beta I tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDaWB result of Beta I tubulin Recombinant Rabbit mAb
Primary antibody: Beta I tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDaWB result of Beta I tubulin Recombinant Rabbit mAb
Primary antibody: Beta I tubulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Beta I tubulin antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Beta I tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- Beta I tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Beta I tubulin in tumor tissue.
Expression of Beta I tubulin in human tissue.
Expression of Beta I tubulin in mouse & rat tissue.
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