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Rabbit抗原名称
TAZ分子别名
WW domain-containing transcription regulator protein 1; Transcriptional coactivator with PDZ-binding motif; TAZ s; WWTR1免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, Cell membraneAccession
Q9GZV5克隆号
S-2812-102抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
A549, HepG2, HeLa, NIH/3T3, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Mk IHC-P 1:1000 Hu ICC 1:50 Hu, Ms ChIP 1:20-1:50 Hu
TAZ (Transcriptional Co-activator with PDZ-binding motif), a 43 kDa paralog of YAP, is a mechanosensitive transcriptional co-activator that shuttles between the cytoplasm and nucleus to drive proliferation, stemness and epithelial-mesenchymal transition by partnering primarily with TEAD factors; it is negatively controlled by the Hippo kinases LATS1/2 through phosphorylation-dependent degradation, but can be de-phosphorylated and activated by PP1/ASPP2 or via GPCR-Rho signaling, and its chronic hyper-activation is strongly linked to tumor progression, metastasis and therapy resistance in multiple solid cancers.
免疫印迹
WB result of TAZ Recombinant Rabbit mAb
Primary antibody: TAZ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 52 kDaWB result of TAZ Recombinant Rabbit mAb
Primary antibody: TAZ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 52 kDaWB result of TAZ Recombinant Rabbit mAb
Primary antibody: TAZ Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 52 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TAZ antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-TAZ antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-TAZ antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TAZ antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-TAZ antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-TAZ antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
Parallel reactions used TAZ Recombinant Rabbit mAb (S-2812-102) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of SMYD3, CCN2 and SAT-α in TAZ Recombinant
Rabbit mAb (S-2812-102)-immunoprecipitated sample.
组织表达图谱
Expression of TAZ in tumor tissue.
Expression of TAZ in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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