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Rabbit抗原名称
SLC25A22分子别名
Mitochondrial glutamate carrier 1; GC-1; Glutamate/H(+) symporter 1; Solute carrier family 25 member 22; GC1免疫原
Recombinant Protein细胞定位
MitochondrionAccession
Q9H936克隆号
S-2780-15抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
PANC-1, HT-29, Caco-2, A431, HepG2, NIH/3T3, mouse brain, PC-12, rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IHC-P 1:100 Hu ICC 1:100 Hu
SLC25A22 is a mitochondrial inner-membrane carrier that exchanges glutamate for H+ across the membrane, thereby providing the matrix with the key anaplerotic substrate needed to sustain the TCA cycle and amino-acid metabolism in neurons and β-cells; mutations in this gene abolish transport activity, cause autosomal-recessive early infantile epileptic encephalopathy-3 (EIEE3) with suppression-burst patterns, and are emerging as drivers of certain cancers by disrupting redox and metabolic homeostasis.
免疫印迹
WB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PANC-1 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: Caco-2 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDaWB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDaWB result of SLC25A22 Recombinant Rabbit mAb
Primary antibody: SLC25A22 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 30 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SLC25A22 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HT-29 cells. Anti- SLC25A22 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SLC25A22 in tumor tissue.
Expression of SLC25A22 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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