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Rabbit抗原名称
PLCγ2分子别名
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; Phosphoinositide phospholipase C-gamma-2; Phospholipase C-IV (PLC-IV); Phospholipase C-gamma-2 (PLC-gamma-2); PLCG2免疫原
Synthetic PeptideAccession
P16885克隆号
S-2749-14抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Ramos, Raji, A20, mouse spleen, rat spleen纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt ICC 1:500 Hu
PLCγ2 (Phospholipase C gamma-2) is a 1,565-residue signaling enzyme that harbors an N-terminal SH2-SH3-SH2 cassette, a split PH domain, tandem EF hands, a catalytic Y-domain TIM barrel, and a C-terminal tail containing two phosphorylatable tyrosines (Y753/Y759); upon recruitment to phosphorylated ITAM-bearing adaptors (e.g., DAP12, FcRγ) or receptor tyrosine kinases, Syk/BTK family kinases trans-phosphorylate these tyrosines, triggering allosteric relief of an auto-inhibited conformation that enables the lipase to hydrolyze PIP2 into IP3 and DAG, thereby mobilizing Ca²⁺ and activating PKC, while simultaneously serving as a scaffold for assembling signalosomes that drive cytoskeletal remodeling, ROS generation and transcriptional programs essential for osteoclastogenesis, B-cell development, platelet aggregation, and innate immune responses; gain-of-function mutations (e.g., S707F, R665W) produce constitutive PIP2 cleavage and cause immune-dysregulatory diseases such as PLAID, APLAID and cold urticaria, whereas loss-of-function variants underlie antibody deficiency and osteopetrosis, making PLCγ2 a druggable node in inflammation, autoimmunity, and hematologic malignancies.
免疫印迹
WB result of PLCγ2 Recombinant Rabbit mAb
Primary antibody: PLCγ2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 22Rv1 whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Negative control: 22Rv1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 148 kDa
Observed MW: 148 kDaWB result of PLCγ2 Recombinant Rabbit mAb
Primary antibody: PLCγ2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 148 kDa
Observed MW: 148 kDaWB result of PLCγ2 Recombinant Rabbit mAb
Primary antibody: PLCγ2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 148 kDa
Observed MW: 148 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-PLCγ2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Ramos cells (top panel) and negative staining in 22Rv1 cells (below panel). Anti- PLCγ2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PLCγ2 in tumor tissue.
Expression of PLCγ2 in human tissue.
Expression of PLCγ2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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