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宿主来源
Rabbit抗原名称
c-Jun分子别名
Transcription factor Jun; Activator protein 1 (AP1); Proto-oncogene c-Jun; Transcription factor AP-1 subunit Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; JUN免疫原
Synthetic Peptide细胞定位
NucleusAccession
P05412克隆号
S-3105-71抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt阳性样本
Anisomycin treated HeLa cells, Anisomycin treated NIH/3T3 cells, Anisomycin treated C6 cells,纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt ICC 1:500 Hu
Phospho-c-Jun (Ser73) refers to the specific post-translational modification where the transcription factor c-Jun is phosphorylated at its serine 73 residue. As a key component of the Activator Protein-1 (AP-1) transcription complex, c-Jun's activity is tightly regulated by the c-Jun N-terminal kinase (JNK) signaling pathway. Upon cellular exposure to stress signals such as UV radiation, cytokine stimulation, or genotoxic stress, JNK becomes activated through phosphorylation and subsequently phosphorylates c-Jun specifically at Ser73. This phosphorylation event is crucial for enhancing c-Jun's transcriptional activity, significantly potentiating the expression of its target genes that govern critical cellular processes including proliferation, differentiation, apoptosis, and inflammatory responses. Consequently, the phosphorylation level at Ser73 serves as a well-established readout for JNK pathway activation, and researchers commonly detect this modification using phospho-specific antibodies through techniques like Western Blotting to assess cellular stress response intensity and related signal transduction events.
免疫印迹
WB result of Phospho-c-Jun (Ser73) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-c-Jun (Ser73) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μg/mL Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDaWB result of Phospho-c-Jun (Ser73) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-c-Jun (Ser73) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 μg/mL Anisomycin for 35 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDaWB result of Phospho-c-Jun (Ser73) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-c-Jun (Ser73) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 25 μg/mL Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 39 kDa
免疫细胞化学
ICC analysis of NIH/3T3 cells treated with Anisomycin (25μg/ml, 30min) (top panel) and untreated NIH/3T3 cells (below panel). Anti- Phospho-c-Jun (Ser73) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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