分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
Phospho-Chk2 (Thr68)分子别名
Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog (Hucds1; hCds1); Checkpoint kinase 2; CDS1; CHK2; RAD53; CHEK2免疫原
Synthetic Peptide细胞定位
NucleusAccession
O96017克隆号
S-2214-11抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu阳性样本
Camptothecin treated HeLa cells纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu
Phospho-Chk2 (Thr68) is the phosphorylated, active form of checkpoint kinase 2 (Chk2), a serine/threonine protein kinase that acts as a key tumor suppressor and DNA-damage transducer; in response to double-strand breaks the PI3K-like kinase ATM rapidly phosphorylates Chk2 on threonine 68, inducing transient dimerization via phospho-Thr68–FHA domain interactions that license subsequent autophosphorylation in the activation loop, thereby generating fully active Chk2 monomers that enforce cell-cycle arrest by phosphorylating substrates such as p53, BRCA1 and the Cdc25 phosphatases, and whose pathway disruption is frequently observed in cancer.
免疫印迹
WB result of Phospho-Chk2 (Thr68) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Chk2 (Thr68) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 μM camptothecin for 1 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 65 kDa
斑点杂交
Dot blot result of Phospho-Chk2 (Thr68) Recombinant Rabbit mAb
Lane 1: ChK2 (Thr68) phospho peptide
Lane 2: ChK2 unmodified peptide
Primary antibody: Phospho-Chk2 (Thr68) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
评论(0)