FAP Recombinant Rabbit mAb (S-3094-29)
Prolyl endopeptidase FAP,170 kDa melanoma membrane-bound gelatinase,Dipeptidyl peptidase FAP,Fibroblast activation protein alpha (FAPalpha),Gelatine degradation protease FAP,Integral membrane serine protease
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宿主来源
Rabbit抗原名称
FAP分子别名
Prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha (FAPalpha); Gelatine degradation protease FAP; Integral membrane serine protease免疫原
Synthetic Peptide细胞定位
Cytoplasm, Secreted, Cell membraneAccession
Q12884克隆号
S-3094-29抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-MEL-28, WI-38纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:2000-1:4000 Hu
Fibroblast Activation Protein (FAP) is a 97-kDa type-II transmembrane serine protease that is virtually absent from healthy adult tissues but becomes highly and persistently up-regulated on activated fibroblasts during wound healing, fibrosis, arthritis, atherosclerosis and in the stroma of over 90 % of epithelial cancers, where its unique endopeptidase (Gly-Pro-X) and dipeptidyl-peptidase activities drive extracellular-matrix remodelling, immune evasion, M2 macrophage polarization and tumour-cell proliferation, migration and invasion via signalling axes such as ITGB1/PI3K/AKT and interactions with fibronectin, collagen and immune-checkpoint ligands, making it a powerful prognostic biomarker and an attractive therapeutic target across multiple malignancies and fibrotic diseases.
免疫印迹
WB result of FAP Recombinant Rabbit mAb
Primary antibody: FAP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Lane 3: WI-38 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 88 kDa
Observed MW: 90 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-FAP antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of FAP in tumor tissue.
Expression of FAP in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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