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eIF2α Rabbit Polyclonal Antibody

Eukaryotic translation initiation factor 2 subunit 1,Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha,eIF-2A,eIF-2alpha,eIF2-alpha),EIF2A,EIF2S1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6525

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

eIF2α
分子别名

Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha; eIF-2A; eIF-2alpha; eIF2-alpha); EIF2A; EIF2S1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Mitochondrion
Accession

P05198
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

PC-3, MCF7, Ramos, HepG2, NIH/3T3, PC-12, COS-7
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk

背景介绍

eIF2α (eukaryotic initiation factor 2 subunit alpha) is a 35 kDa phosphoprotein that, together with the G-protein βγ dimer eIF2B, forms the heterotrimeric eIF2 complex essential for delivering the initiator methionyl-tRNA to the 40S ribosomal subunit in a GTP-dependent manner; phosphorylation of Ser51 by stress-sensing kinases (PKR, PERK, HRI, GCN2) converts eIF2 into a non-competitive inhibitor of its guanine-nucleotide-exchange factor eIF2B, thereby repressing global cap-dependent translation while selectively permitting ATF4 and other stress-response mRNAs, a switch that underlies cellular adaptation to starvation, oxidative stress, viral infection, and proteotoxicity, and whose dysregulation contributes to diseases ranging from neurodegeneration and cancer to diabetes and viral pathogenesis.

免疫印迹
- WB result of eIF2α Rabbit pAb
  Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
  Lane 1: PC-3 whole cell lysate 20 µg
  Lane 2: MCF7 whole cell lysate 20 µg
  Lane 3: Ramos whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 37 kDa
- WB result of eIF2α Rabbit pAb
  Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 37 kDa
- WB result of eIF2α Rabbit pAb
  Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 37 kDa
- WB result of eIF2α Rabbit pAb
  Primary antibody: eIF2α Rabbit pAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 36 kDa
  Observed MW: 37 kDa