c-Raf Recombinant Rabbit mAb (S-2718-61)

RAF proto-oncogene serine/threonine-protein kinase,Proto-oncogene c-RAF (cRaf),Raf-1,RAF,RAF1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6523

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

c-Raf
分子别名

RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF (cRaf); Raf-1; RAF; RAF1
免疫原

Synthetic Peptide
细胞定位

Cell membrane, Cytoplasm, Nucleus, Mitochondrion
Accession

P04049
克隆号

S-2718-61
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HeLa, A549, C2C12, NIH/3T3, PC-12, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt, Mk
ICC 1:100 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
ICC	1:100	Hu

背景介绍

c-Raf (also called Raf-1) is a 648-amino-acid serine/threonine kinase that serves as the pivotal upstream cytoplasmic MAP3K in the classical Ras→Raf→MEK→ERK/MAPK cascade: upon growth-factor- or oncogenic Ras-GTP-mediated recruitment to the plasma membrane, it homo- or hetero-dimerizes, becomes phosphorylated on multiple activating residues (Ser338, Tyr341, Thr491, Ser494) while relieving N-terminal autoinhibition, and then processively phosphorylates MEK1/2 to trigger downstream proliferative, anti-apoptotic, and differentiative transcriptional programs; its activity is tightly controlled via feedback and scaffold proteins, and gain-of-function mutations, overexpression, or dysregulated upstream signaling of c-Raf are implicated in numerous cancers, making it a key therapeutic target.

免疫印迹
- WB result of c-Raf Recombinant Rabbit mAb
  Primary antibody: c-Raf Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
- WB result of c-Raf Recombinant Rabbit mAb
  Primary antibody: c-Raf Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C2C12 whole cell lysate 20 µg
  Lane 2: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
- WB result of c-Raf Recombinant Rabbit mAb
  Primary antibody: c-Raf Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
- WB result of c-Raf Recombinant Rabbit mAb
  Primary antibody: c-Raf Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 73 kDa
  Observed MW: 68 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti-c-Raf antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).