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Rabbit抗原名称
CD70分子别名
CD70 antigen; CD27 ligand (CD27-L); Tumor necrosis factor ligand superfamily member 7Imported; CD27L; CD27LG; TNFSF7免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P32970克隆号
S-1725-277抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
U266B1, SK-OV-3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu ICC 1:100 Hu
CD70, also known as CD27 ligand, is a type Ⅱ transmembrane protein that plays a crucial role in the immune system. It is primarily expressed on antigen - presenting cells (APCs) such as dendritic cells, macrophages, and B cells, as well as on activated T cells. CD70 interacts with its receptor CD27, which is mainly found on T cells, B cells, and natural killer (NK) cells. This interaction provides important co - stimulatory signals that promote T cell activation, proliferation, and differentiation, and also enhances B cell and NK cell functions. Additionally, CD70 is involved in the regulation of immune responses, including the maintenance of immune homeostasis and the induction of immune - mediated tumor cell killing. Abnormal expression of CD70 has been associated with various diseases, such as autoimmune disorders and certain types of cancers. Therefore, CD70 is considered a promising target for the development of immunotherapies.
免疫印迹
WB result of CD70 Recombinant Rabbit mAb
Primary antibody: CD70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: U266B1 whole cell lysate 20 µg
Lane 5: SK-OV-3 whole cell lysate 20 µg
Low expression control: Jurkat whole cell lysate; SH-SY5Y whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 25, 54, 85 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adneocarcinoma. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in U266B1 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD70 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CD70 in tumor tissue.
Expression of CD70 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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