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PIN1 Recombinant Rabbit mAb (S-2731-43)

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1,Peptidyl-prolyl cis-trans isomerase Pin1 (PPIase Pin1),Rotamase Pin1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6515

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

PIN1
分子别名

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1 (PPIase Pin1); Rotamase Pin1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13526
克隆号

S-2731-43
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HEK-293, Jurkat, A549, HeLa, NIH/3T3, mouse heart, PC-12, rat brain, COS-7
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt, Mk
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
ICC	1:500	Hu

背景介绍

PIN1 is a conserved 163-residue peptidyl-prolyl cis-trans isomerase composed of an N-terminal WW domain and a C-terminal catalytic PPIase domain, both recognizing pSer/Thr-Pro motifs; by selectively isomerizing these phosphorylated bonds it acts as a post-phosphorylation switch that reshapes the conformation, stability, localization and ubiquitination of hundreds of client proteins—ranging from p53 and cyclins to NF-κB and APP—thereby governing cell-cycle progression, transcription, immune signaling and neuronal homeostasis, while its frequent over-activation in cancer drives oncogenic pathways and its loss contributes to neurodegenerative diseases such as Alzheimer’s.

免疫印迹
- WB result of PIN1 Recombinant Rabbit mAb
  Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: A549 whole cell lysate 20 µg
  Lane 4: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 18 kDa
- WB result of PIN1 Recombinant Rabbit mAb
  Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 18 kDa
- WB result of PIN1 Recombinant Rabbit mAb
  Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat brain lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 18 kDa
- WB result of PIN1 Recombinant Rabbit mAb
  Primary antibody: PIN1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 18 kDa
  Observed MW: 18 kDa
免疫细胞化学
- ICC shows positive staining in HEK293 cells. Anti- PIN1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).