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Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb (S-2765-18)

Glycogen [starch] synthase,muscle,Glycogen synthase 1,GYS,GYS1

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B6514
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产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Phospho-Glycogen Synthase (Ser641)
  • 分子别名

    Glycogen [starch] synthase, muscle; Glycogen synthase 1; GYS; GYS1
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Cytoplasm
  • Accession

    P13807
  • 克隆号

    S-2765-18
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 翻译后修饰类型

    磷酸化
  • 反应种属 ?

    Hu, Ms, Rt
  • 阳性样本

    HeLa, NIH/3T3, PC-12
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt
背景介绍
  • Phospho-Glycogen Synthase (Ser641) is the inactive, phosphorylated form of glycogen synthase in which a phosphate group is covalently attached to serine-641; this single modification, catalyzed chiefly by glycogen synthase kinase-3 (GSK-3) downstream of insulin signaling, sterically hinders the enzyme’s active site and prevents the elongation of glycogen chains, thereby providing a rapid, reversible switch that links hormonal cues to systemic glucose storage.

  • 免疫印迹

    • WB result of Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HeLa whole cell lysate 20 µg (λ phosphatase treated membrane)
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 84 kDa
      Observed MW: 90 kDa

    • WB result of Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg (λ phosphatase treated membrane)
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 84 kDa
      Observed MW: 90 kDa

    • WB result of Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Glycogen Synthase (Ser641) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg (λ phosphatase treated membrane)
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 84 kDa
      Observed MW: 90 kDa

FAQs

斯达特公司的抗体,可以回收利用几次?

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定

检测磷酸化蛋白时,推荐有什么封闭体系?

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗,进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂,但是脱脂奶粉获取更加方便,覆盖更广泛的非特异性结合位点,在一抗性能优越的前提下,使用脱脂奶粉封闭性价比更高

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