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VDAC1 + VDAC2 + VDAC3 Rabbit Polyclonal Antibody

Voltage-dependent anion-selective channel protein,hVDAC1,Non-selective voltage-gated ion channel VDAC2,VDAC-2,Non-selective voltage-gated ion channel VDAC3,VDAC-3,hVDAC3,Outer mitochondrial membrane protein porin 3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6511

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

VDAC1 + VDAC2 + VDAC3
分子别名

Voltage-dependent anion-selective channel protein; hVDAC1; Non-selective voltage-gated ion channel VDAC2; VDAC-2; Non-selective voltage-gated ion channel VDAC3; VDAC-3; hVDAC3; Outer mitochondrial membrane protein porin 3
免疫原

Synthetic Peptide
细胞定位

Endoplasmic reticulum, Mitochondrion, Cell membrane
Accession

P21796, Q9MZ15, Q9Y277
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

HeLa, A431, Jurkat, HEK-293, HepG2, NIH/3T3, mouse heart, PC-12, rat heart
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:2000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

背景介绍

VDAC1, VDAC2, and VDAC3 are three closely related ~30 kDa β-barrel porins embedded in the mitochondrial outer membrane where they form voltage-dependent anion channels that collectively serve as the primary gatekeepers for metabolite flux—allowing ATP/ADP, NADH, ion, and other small-molecule exchange between cytosol and mitochondria—while also acting as hubs for Ca²⁺ signaling, apoptotic protein binding, and interaction with hexokinase, creatine kinase, Bcl-2 family proteins, and cytoskeletal elements; despite high sequence similarity, isoforms show tissue-specific expression (VDAC1 ubiquitous, VDAC2 enriched in heart/testis and uniquely anti-apoptotic via Bax inhibition, VDAC3 prominent in testis and cilia with redox-sensitive cysteines linked to sperm motility and ROS regulation), distinct electrophysiological gating properties, and differential roles in oxidative stress, mitochondrial dynamics, and apoptosis, making the trio essential for bioenergetics, cell survival decisions, and increasingly recognized as therapeutic targets in cancer, neurodegeneration, and metabolic disorders.

免疫印迹
- WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
  Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A431 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: HEK-293 whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 32 kDa
- WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
  Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 32 kDa
- WB result of VDAC1 + VDAC2 + VDAC3 Rabbit pAb
  Primary antibody: VDAC1 + VDAC2 + VDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: rat heart lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 31 kDa
  Observed MW: 32 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-VDAC1 + VDAC2 + VDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of VDAC1 + VDAC2 + VDAC3 in tumor tissue
- Expression of VDAC1 + VDAC2 + VDAC3 in human tissue
- Expression of VDAC1 + VDAC2 + VDAC3 in mouse & rat tissue