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UQCRC2 Recombinant Rabbit mAb (S-1890-227)

Cytochrome b-c1 complex subunit 2,mitochondrial,Complex III subunit 2,Core protein II,Ubiquinol-cytochrome-c reductase complex core protein 2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6510

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

UQCRC2
分子别名

Cytochrome b-c1 complex subunit 2, mitochondrial; Complex III subunit 2; Core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

P22695
克隆号

S-1890-227
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, HepG2, Ramos, HCT 116, Neuro-2a, mouse heart, C6, rat heart
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Rt
IHC-P 1:200 Hu, Ms, Rt
ICC 1:100 Hu

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:200	Hu, Ms, Rt
ICC	1:100	Hu

背景介绍

UQCRC2 (ubiquinol-cytochrome c reductase core protein 2), encoded on chromosome 16p12.2, is a 453-amino-acid, 48.4 kDa integral component of mitochondrial complex III (cytochrome b-c1 complex) that stabilizes the ~450 kDa multisubunit transmembrane assembly and, by facilitating electron flow from ubiquinol to cytochrome c coupled to proton pumping across the inner membrane, powers the electrochemical gradient essential for oxidative phosphorylation, while its peptidase-M16-family processing activity contributes to maturation of the Rieske iron-sulfur protein and mutations in UQCRC2 are linked to mitochondrial complex III deficiency nuclear type 5.

免疫印迹
- WB result of UQCRC2 Recombinant Rabbit mAb
  Primary antibody: UQCRC2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HepG2 whole cell lysate 20 µg
  Lane 3: Ramos whole cell lysate 20 µg
  Lane 4: HCT 116 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
- WB result of UQCRC2 Recombinant Rabbit mAb
  Primary antibody: UQCRC2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Neuro-2a whole cell lysate 20 µg
  Lane 2: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
- WB result of UQCRC2 Recombinant Rabbit mAb
  Primary antibody: UQCRC2 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat heart lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 48 kDa
  Observed MW: 48 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human liver. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-UQCRC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti- UQCRC2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of UQCRC2 in tumor tissue.
- Expression of UQCRC2 in human tissue.
- Expression of UQCRC2 in mouse & rat tissue.