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Rabbit抗原名称
SCAMP1分子别名
Secretory carrier-associated membrane protein 1; Secretory carrier membrane protein 1; SCAMP免疫原
Recombinant Protein细胞定位
EndosomeAccession
O15126抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, SH-SY5Y, U-87 MG, MCF7, Neuro-2a, mouse brain, mouse pancreas, rat brain预测反应种属
(反应种属缩写表)Pg纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:200 Hu, Ms, Rt
SCAMP1 (Secretory Carrier Membrane Protein 1) is a ubiquitously expressed, four-pass transmembrane protein that cycles between the trans-Golgi network, endosomes and the plasma membrane, where it acts as a multifunctional regulator of post-Golgi membrane trafficking, exocytosis and cell-surface recycling; its cytosolic N- and C-terminal domains contain NPF motifs, a proline-rich SH3-binding site and three conserved amphiphilic segments that dock at the cytoplasmic membrane interface, enabling SCAMP1 to nucleate protein–protein interactions with SNAREs and other trafficking factors, thereby facilitating secretory granule exocytosis in neuroendocrine cells and insulin-secreting β-cells, while in epithelia it cooperates with the metastasis suppressor MTSS1 to stabilize Rac1-based cell–cell adhesions and curb cancer invasion, and palmitoylation of its cytosolic segments dynamically modulates these membrane exchange and anti-invasive functions.
免疫印迹
WB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDaWB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDaWB result of SCAMP1 Rabbit pAb
Primary antibody: SCAMP1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SCAMP1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SCAMP1 in tumor tissue.
Expression of SCAMP1 in human tissue.
Expression of SCAMP1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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