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Rabbit抗原名称
PBEF/Visfatin/NAMPT分子别名
Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1 (Pre-B cell-enhancing factor); Visfatin; PBEF1免疫原
Recombinant Protein细胞定位
Cytoplasm, Nucleus, SecretedAccession
P43490抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu阳性样本
HeLa, HT-1080, K562, MCF7, PC-3纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu ICC 1:500 Hu
PBEF/Visfatin/NAMPT is a multifunctional 52-kDa protein that acts both intracellularly as the rate-limiting enzyme nicotinamide phosphoribosyltransferase (EC 2.4.2.12) in the NAD salvage pathway—catalyzing conversion of nicotinamide to nicotinamide mononucleotide (NMN)—and extracellularly as an adipokine/cytokine circulating in blood; it enhances B-lymphocyte precursor maturation, inhibits neutrophil apoptosis, binds and activates the insulin receptor to exert insulin-mimetic glucose-lowering effects, promotes vascular smooth-muscle cell maturation, modulates endothelial function, and its serum levels correlate with obesity and metabolic syndrome, while intracellularly it maintains the NAD⁺ pool essential for processes such as SIRT1 activity, DNA repair and β-cell glucose-stimulated insulin secretion.
免疫印迹
WB result of PBEF/Visfatin/NAMPT Rabbit pAb
Primary antibody: PBEF/Visfatin/NAMPT Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HT-1080 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 54 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-PBEF/Visfatin/NAMPT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of PBEF/Visfatin/NAMPT in tumor tissue.
Expression of PBEF/Visfatin/NAMPT in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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