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NMT1 Recombinant Rabbit mAb (S-2986-17)

Glycylpeptide N-tetradecanoyltransferase 1,Myristoyl-CoA:protein N-myristoyltransferase 1 (HsNMT1,NMT 1,Type I N-myristoyltransferase),Peptide N-myristoyltransferase 1,Protein-lysine myristoyltransferase NMT1,NMT

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6504

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

NMT1
分子别名

Glycylpeptide N-tetradecanoyltransferase 1; Myristoyl-CoA:protein N-myristoyltransferase 1 (HsNMT1; NMT 1; Type I N-myristoyltransferase); Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; NMT
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

P30419
克隆号

S-2986-17
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

SK-OV-3, HeLa, NIH/3T3, mouse brain, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000-1:2000 Hu, Ms, Mk
IHC-P 1:1000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000-1:2000	Hu, Ms, Mk
IHC-P	1:1000	Hu, Ms, Rt

背景介绍

NMT1 is a cytosolic monomeric enzyme that catalyzes the irreversible, co-translational transfer of myristic acid from myristoyl-CoA to the N-terminal glycine of nascent polypeptides, a modification essential for membrane targeting, signal transduction, and stability of oncogenic kinases (e.g., Src, FAK), and its overexpression in cancers promotes proliferation, suppresses autophagy, and supports metabolic reprogramming through specific regulation of AMPKβ myristoylation, making it a validated therapeutic target.

免疫印迹
- WB result of NMT1 Recombinant Rabbit mAb
  Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: SK-OV-3 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 60 kDa
- WB result of NMT1 Recombinant Rabbit mAb
  Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 60 kDa
- WB result of NMT1 Recombinant Rabbit mAb
  Primary antibody: NMT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat testis. Anti-NMT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of NMT1 in tumor tissue.
- Expression of NMT1 in human tissue.
- Expression of NMT1 in mouse & rat tissue.