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Rabbit抗原名称
Atg3分子别名
Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3 (APG3-like; hApg3); Protein PC3-96; APG3; APG3L; ATG3免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
Q9NT62克隆号
S-2168-20抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HL-60, K562, THP-1, HeLa, A20, mouse kidney, PC-12, COS-7预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu ICC 1:100 Hu
Atg3 is an E2-like enzyme essential for autophagosome biogenesis: its C-terminal catalytic cysteine forms a thioester bond with the C-terminal glycine of Atg8-family proteins (LC3/GABARAPs) in an ATP-dependent manner, and subsequently, with the E3-like Atg12–Atg5-Atg16L1 complex, it covalently conjugates Atg8 to phosphatidylethanolamine on the expanding phagophore membrane, thereby driving membrane curvature, expansion, and closure into the double-membrane autophagosome that sequesters cytoplasmic cargo for lysosomal degradation.
免疫印迹
WB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDaWB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDaWB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDaWB result of Atg3 Recombinant Rabbit mAb
Primary antibody: Atg3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 38 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Atg3 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- Atg3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Atg3 in tumor tissue.
Expression of Atg3 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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