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Rabbit抗原名称
Granzyme K分子别名
Fragmentin-3; Granzyme-3; NK-tryptase-2 (NK-Tryp-2); TRYP2; GZMK免疫原
Synthetic Peptide细胞定位
SecretedAccession
P49863克隆号
S-2665-22抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HuT-78, NK-92纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Granzyme K is a serine protease stored in the cytotoxic granules of CD8⁺ T cells and NK cells that, upon perforin-mediated entry into target cells, cleaves the nucleosome-assembly factor SET and the DNA damage-response factor Ku70 to trigger caspase-independent nuclear condensation, mitochondrial perturbation, and rapid cell death, thereby amplifying antiviral and antitumor immunity.
免疫印迹
WB result of Granzyme K Recombinant Rabbit mAb
Primary antibody: Granzyme K Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HuT-78 whole cell lysate 20 µg
Lane 4: NK-92 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; SH-SY5Y whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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