GRP75 Rabbit Polyclonal Antibody
Stress-70 protein,mitochondrial,75 kDa glucose-regulated protein (GRP-75),Heat shock 70 kDa protein 9,Heat shock protein family A member 9,Mortalin (MOT),Peptide-binding protein 74 (PBP74),HSPA9B,mt-HSP70,HSPA9
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宿主来源
Rabbit抗原名称
GRP75分子别名
Stress-70 protein, mitochondrial; 75 kDa glucose-regulated protein (GRP-75); Heat shock 70 kDa protein 9; Heat shock protein family A member 9; Mortalin (MOT); Peptide-binding protein 74 (PBP74); HSPA9B; mt-HSP70; HSPA9免疫原
Recombinant Protein细胞定位
Cytoplasm, Nucleus, Mitochondrion, Endoplasmic reticulumAccession
P38646抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
MCF7, HeLa, PANC-1, Jurkat, K562, NIH/3T3, mouse liver, PC-12, rat brain, COS-7纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu
GRP75, also known as mortalin or HSPA9, is an evolutionarily highly-conserved 75-kDa heat-shock protein that resides mainly in mitochondria but also shuttles to cytosol, ER, plasma membrane and even nucleus; it functions as a multifunctional molecular chaperone that binds unfolded or misfolded proteins in an ATP-dependent manner to prevent aggregation, drives import and proper folding of nascent mitochondrial precursors, participates in mitochondrial biogenesis, iron–sulfur cluster assembly, calcium homeostasis, and quality-control proteolysis, and additionally regulates stress response, cell-cycle progression, senescence and apoptosis by modulating p53, telomerase and other signaling pathways; consequently, its overexpression is frequently observed in many tumors where it promotes survival and chemoresistance, whereas its loss-of-function mutations cause severe neurodegenerative and mitochondrial disorders, making GRP75 a pivotal hub linking bioenergetics, proteostasis and human disease.
免疫印迹
WB result of GRP75 Rabbit pAb
Primary antibody: GRP75 Rabbit pAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74 kDaWB result of GRP75 Rabbit pAb
Primary antibody: GRP75 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74 kDaWB result of GRP75 Rabbit pAb
Primary antibody: GRP75 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74 kDaWB result of GRP75 Rabbit pAb
Primary antibody: GRP75 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 74 kDa
Observed MW: 74 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-GRP75 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GRP75 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GRP75 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GRP75 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-GRP75 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti-GRP75 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of GRP75 in tumor tissue.
Expression of GRP75 in human tissue.
Expression of GRP75 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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