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Rabbit抗原名称
FIS1分子别名
Mitochondrial fission 1 protein; FIS1 homolog (hFis1); Tetratricopeptide repeat protein 11 (TPR repeat protein 11); TTC11免疫原
Recombinant Protein细胞定位
MitochondrionAccession
Q9Y3D6抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK-293, HeLa, Jurkat, HepG2, A431, EL4, PC-12, rat spleen, COS-7纯化方式
Immunogen Affinity浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:2000 Hu, Ms, Rt, Mk
FIS1 (Mitochondrial Fission Protein 1) is a 17-kDa outer mitochondrial membrane protein that plays a central role in mitochondrial quality control by promoting organellar fission: under stress it recruits the GTPase DRP1 to form a constriction ring that severs mitochondria, while also inhibiting fusion proteins (MFN/OPA1), engaging mitophagy adaptors (TBC1D15/17) to clear damaged fragments, and interacting with BAP31 to amplify apoptotic signals, thereby governing mitochondrial morphology, energy metabolism, cell survival and—when dysregulated—tumorigenesis or neurodegeneration.
免疫印迹
WB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDaWB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDaWB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDaWB result of FIS1 Rabbit pAb
Primary antibody: FIS1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 17 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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