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Rabbit抗原名称
Cleaved Caspase-3 (Asp175)分子别名
Caspase-3; CASP-3; Apopain; Cysteine protease CPP32 (CPP-32); Protein Yama; SREBP cleavage activity 1 (SCA-1); CPP32; CASP3细胞定位
CytoplasmAccession
P42574克隆号
S-2817抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 50% Glycerol, 0.05%BSA,0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms ICC 1:500 Hu
Cleaved Caspase-3 (Asp175) is the ~17/19 kDa proteolytically activated form of human caspase-3 that, once cleaved after Asp175-Ser176 during apoptosis, loses its N-terminal pro-domain, assembles into active hetero-tetramers (p17/p12) and relentlessly hydrolyzes Asp-x bonds in >100 cytoplasmic, nuclear and cytoskeletal substrates—such as PARP, ICAD, ROCK1 and gelsolin—thereby dismantling cellular architecture, inactivating DNA repair, triggering chromatin condensation, membrane blebbing and phosphatidyl-serine exposure, and thus serving as the pivotal executioner caspase whose appearance, detected by the widely used “Asp175” neo-epitope antibody, is universally recognized as an irreversible biochemical signature of cells committed to programmed cell death.
免疫印迹
WB result of Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 1 μM Staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 17, 19 kDaWB result of Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb
Primary antibody: Cleaved Caspase-3 (Asp175) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 1 μM Staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 17, 19 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse embryo. Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC analysis of HeLa cells starve overnight then treated with Staurosporine (1μM,3 hours) (top panel) and untreated HeLa cells (below panel). Anti-Cleaved Caspase-3 (Asp175) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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