VE-Cadherin Recombinant Rabbit mAb (S-1291-239)

Cadherin-5,7B4 antigen,Vascular endothelial cadherin (VE-cadherin),CDH5

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6493

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CD144
分子别名

Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Cell membrane
Accession

P33151
克隆号

S-1291-239
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
阳性样本

mouse heart, rat heart
预测反应种属
(反应种属缩写表)

Pg, Ck
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IHC-P 1:2000 Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:2000	Ms, Rt

背景介绍

CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation.

免疫印迹
- WB result of VE-Cadherin Recombinant Rabbit mAb
  Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 88 kDa
  Observed MW: 130 kDa
- WB result of VE-Cadherin Recombinant Rabbit mAb
  Primary antibody: VE-Cadherin Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat heart lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 88 kDa
  Observed MW: 130 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded mouse lung. Anti- VE-Cadherin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti- VE-Cadherin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of VE-Cadherin in mouse & rat tissue.