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宿主来源
Rabbit抗原名称
StAR分子别名
Steroidogenic acute regulatory protein, mitochondrial; START domain-containing protein 1 (StARD1); STARD1; STAR免疫原
Synthetic Peptide细胞定位
MitochondrionAccession
P49675克隆号
S-2520-24抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Rt阳性样本
K562纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu, Rt
StAR (Steroidogenic Acute Regulatory) protein is a 37-kDa mitochondrial phosphoprotein that catalyzes the rate-limiting step in steroid hormone biosynthesis by ferrying cholesterol from the outer to the inner mitochondrial membrane; upon tropic hormone stimulation its N-terminal 25-amino-acid mitochondrial targeting sequence is cleaved to yield the 30-kDa mature form, and mutations in the STAR gene cause the potentially fatal lipoid congenital adrenal hyperplasia in humans.
免疫印迹
WB result of StAR Recombinant Rabbit mAb
Primary antibody: StAR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; Ramos whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 32 kDa
Observed MW: 25 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human adrenal gland. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pheochromocytoma. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-StAR antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of StAR in tumor tissue.
Expression of StAR in human tissue.
Expression of StAR in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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