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Rabbit抗原名称
SMARCAL1分子别名
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein (hHARP); Sucrose nonfermenting protein 2-like 1; HARP s免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9NZC9克隆号
S-2110-328抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HEK-293, A549, HeLa, A431, Jurkat, NIH/3T3, PC-12, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:50 Hu, Ms
SMARCAL1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like 1) is a DNA-stimulated ATPase and annealing helicase that travels with elongating replication forks and, upon detecting replication stress or DNA damage, catalyzes fork regression to form a protective “chicken-foot” structure and subsequent fork restoration, thereby stabilizing stalled forks, promoting template switching and homology-directed repair, and preventing MUS81-mediated double-strand breaks, while its tight regulation by RPA binding orientation and ATR-dependent phosphorylation of Ser-652 ensures controlled remodeling activity essential for maintaining genome stability, and germline mutations that abolish these functions cause the multisystem disorder Schimke immuno-osseous dysplasia.
免疫印迹
WB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDaWB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDaWB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-120 kDaWB result of SMARCAL1 Recombinant Rabbit mAb
Primary antibody: SMARCAL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 106 kDa
Observed MW: 90-150 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SMARCAL1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of SMARCAL1 in tumor tissue.
Expression of SMARCAL1 in human tissue.
Expression of SMARCAL1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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