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Phospho-Rb (Ser780) Recombinant Mouse mAb (S-3521)

Retinoblastoma-associated protein,p105-Rb,p110-RB1,pRb (Rb),pp110,RB1

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6486
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 抗原名称

    Phospho-Rb (Ser780)

  • 分子别名

    Retinoblastoma-associated protein; p105-Rb; p110-RB1; pRb (Rb); pp110; RB1

  • 细胞定位

    Nucleus, Cytoplasm

  • Accession

    P06400

  • 克隆号

    S-3521

  • 抗体类型

    Mouse mAb

  • 抗体同种型

    IgG1,k

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Mk

  • 阳性样本

    Jurkat, Molt-4, COS-7

  • 纯化方式

    Protein G

  • 浓度

    2 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Mk
背景介绍

  • Phospho-Rb (Ser780) refers to the specific modification form of the retinoblastoma protein (Rb) phosphorylated at its serine 780 residue. The Rb protein is a key regulator of the cell cycle G1/S checkpoint. In its unphosphorylated state, it halts cell cycle progression by binding to and inhibiting E2F transcription factors. When cells receive proliferative signals, the cyclin-dependent kinase 4/6-cyclin D complex specifically catalyzes the phosphorylation of the Rb protein at the Ser780 site. This event is an early and critical step in the inactivation process of the Rb protein. Phosphorylation at Ser780 directly disrupts the interaction between Rb and E2F, thereby relieving the inhibition. The released E2F then activates the transcription of a suite of genes necessary for S-phase entry, driving the cell cycle forward. Consequently, detecting the level of Phospho-Rb (Ser780) is commonly used to assess CDK4/6 activity and the state of cell proliferation, holding significant importance in cancer research and the development of targeted therapies, such as CDK4/6 inhibitors.

  • 免疫印迹

    • WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
      Lane 1: Jurkat whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 106 kDa
      Observed MW: 110 kDa

    • WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
      Lane 1: Molt-4 treated with 500 ng/ml Ionomycin and 10 ng/m PMA for 24 hours whole cell lysate 20 µg
      Lane 2: Molt-4 treated with 500 ng/ml Ionomycin and 10 ng/m PMA for 24 hours whole cell lysate 20 µg (phosphatase treated membrane)
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 106 kDa
      Observed MW: 110 kDa

    • WB result of Phospho-Rb (Ser780) Recombinant Mouse mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: Phospho-Rb (Ser780) Recombinant Mouse mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 106 kDa
      Observed MW: 110 kDa

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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