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Rabbit抗原名称
Phospho-Akt Substrate (RXRXXST)免疫原
Synthetic Peptide克隆号
S-2624-20抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ELISA
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
The Phospho-Akt Substrate (RXRXXS/T) motif is the core sequence specifically recognized and phosphorylated by protein kinase B (Akt). In this motif, R represents arginine, X represents any amino acid, and S/T represents the phosphorylation site serine or threonine. The characteristic arginine residues (RXR) are responsible for specific binding to the catalytic domain of Akt, while the C-terminal serine or threonine (S/T) is the site where Akt catalyzes the phosphorylation reaction. This highly conserved motif is widely found in various downstream substrate proteins of Akt, such as GSK-3β and FOXO transcription factors, and serves as a central hub regulating key signaling pathways involved in cell survival, proliferation, and metabolism. Detecting the phosphorylation level of this motif directly reflects the in vivo activity status of Akt kinase.
免疫印迹
WB result of Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: MultipleWB result of Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-Akt Substrate (RXRXXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 ng/ml Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
ELISA
ELISA binding assay shows Phospho-Akt Substrate (RXRXXST) Antibody specifically binds to phospho and non-phospho motif peptides.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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