分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
ORAI3分子别名
Protein orai-3; Transmembrane protein 142C; TMEM142C免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9BRQ5克隆号
S-2486-117抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
NIH/3T3, RAW264.7, A20纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
ORAI3 is a four-transmembrane store-operated Ca²⁺ channel protein that, upon association with the endoplasmic-reticulum Ca²⁺ sensor STIM1, forms highly Ca²⁺-selective CRAC channels mediating store-operated Ca²⁺ entry (SOCE) essential for T- and B-cell activation, endothelial and smooth-muscell function, and metabolic reprogramming; beyond SOCE, ORAI3 can heteromerize with ORAI1 to create arachidonic-acid-activated, store-independent channels, is transcriptionally up-regulated by TGF-β to drive the epithelial–mesenchymal transition gene Snai1, and in many cancers its over-expression promotes stemness, migration, chemoresistance and tumour growth via both Ca²⁺-dependent and Ca²⁺-independent signalling, making it a potential prognostic biomarker and therapeutic target.
免疫印迹
WB result of ORAI3 Recombinant Rabbit mAb
Primary antibody: ORAI3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 28 kDa
This blot was developed with high sensitivity substrate
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)