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NF-κB2 p100 Recombinant Rabbit mAb (S-2502-62)

Nuclear factor NF-kappa-B p100 subunit,DNA-binding factor KBF2,H2TF1,Lymphocyte translocation chromosome 10 protein,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2,Oncogene Lyt-10 (Lyt10),LYT10,NFKB2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6482

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

NF-κB2 p100
分子别名

Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10 (Lyt10); LYT10; NFKB2
免疫原

Recombinant Protein
细胞定位

Cytoplasm, Nucleus
Accession

Q00653
克隆号

S-2502-62
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Mk
阳性样本

HeLa, HDLM-2, Karpas-299, MCF7, THP-1, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Mk
IHC-P 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Mk
IHC-P	1:50	Hu

背景介绍

NF-κB2 p100 is a 100 kDa cytoplasmic precursor protein encoded by the nfkb2 gene that functions both as an IκB-like inhibitor of NF-κB—sequestering RelB and other subunits in the cytoplasm—and as the obligate source of the mature NF-κB2 p52 subunit generated via stimulus-induced, phosphorylation- and ubiquitin-dependent partial proteasomal processing triggered by the alternative NF-κB pathway kinases NIK and IKKα, thereby enabling p52-RelB dimers to translocate to the nucleus and drive transcriptional programs essential for lymphoid organogenesis, B-cell maturation and adaptive immunity.

免疫印迹
- WB result of NF-κB2 p100 Recombinant Rabbit mAb
  Primary antibody: NF-κB2 p100 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: HDLM-2 whole cell lysate 20 µg
  Lane 3: Karpas-299 whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Lane 5: THP-1 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 97 kDa
  Observed MW: 110 kDa
- WB result of NF-κB2 p100 Recombinant Rabbit mAb
  Primary antibody: NF-κB2 p100 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 97 kDa
  Observed MW: 110 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NF-κB2 p100 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of NF-κB2 p100 in tumor tissue.
- Expression of NF-κB2 p100 in human tissue.