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Rabbit抗原名称
HLA-G分子别名
HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; MHC class I antigen G; HLA-6.0; HLAG免疫原
Recombinant Protein细胞定位
Cell membrane, Endoplasmic reticulum, Secreted, EndosomeAccession
P17693克隆号
S-2711-78抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-OV-3, U937, Jurkat, JEG-3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:2000-1:10000 Hu
HLA-G (human leukocyte antigen-G) is a non-classical, heavily glycosylated HLA class Ib molecule that is selectively expressed at the maternal-fetal interface (primarily on invading extravillous cytotrophoblasts) and in immune-privileged adult tissues, where it exerts potent tolerogenic functions through interaction with inhibitory receptors (ILT2, ILT4, KIR2DL4) on NK cells, T cells, macrophages and dendritic cells, thereby inhibiting cytotoxicity, proliferation and cytokine production, promoting regulatory T-cell generation, and ultimately contributing to immune evasion by tumors, acceptance of semi-allogeneic fetuses, and survival of transplanted grafts; its surface expression can be generated through alternative splicing of seven reported isoforms (HLA-G1 to -G7), with HLA-G1 being the full-length membrane-bound form that can also be proteolytically cleaved to generate soluble HLA-G5, while the other isoforms are either soluble or anchored via glycolipid, and its expression is tightly regulated by genetically polymorphic 5′-upstream regulatory elements, hypoxia, cytokines (IL-10, IFN-β, GM-CSF), and microRNAs, making HLA-G a key diagnostic, prognostic and therapeutic target in pregnancy complications, transplantation, autoimmunity and cancer.
免疫印迹
WB result of HLA-G Recombinant Rabbit mAb
Primary antibody: HLA-G Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: U937 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: JEG-3 whole cell lysate 20 µg
Negative control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 38, 40 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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