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HLA-G Recombinant Rabbit mAb (S-2711-78)

HLA class I histocompatibility antigen,alpha chain G,HLA G antigen,MHC class I antigen G,HLA-6.0,HLAG

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B6480
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产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    HLA-G
  • 分子别名

    HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; MHC class I antigen G; HLA-6.0; HLAG
  • 免疫原

    Recombinant Protein
  • 细胞定位

    Cell membrane, Endoplasmic reticulum, Secreted, Endosome
  • Accession

    P17693
  • 克隆号

    S-2711-78
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 反应种属 ?

    Hu
  • 阳性样本

    SK-OV-3, U937, Jurkat, JEG-3
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:2000-1:10000 Hu
背景介绍
  • HLA-G (human leukocyte antigen-G) is a non-classical, heavily glycosylated HLA class Ib molecule that is selectively expressed at the maternal-fetal interface (primarily on invading extravillous cytotrophoblasts) and in immune-privileged adult tissues, where it exerts potent tolerogenic functions through interaction with inhibitory receptors (ILT2, ILT4, KIR2DL4) on NK cells, T cells, macrophages and dendritic cells, thereby inhibiting cytotoxicity, proliferation and cytokine production, promoting regulatory T-cell generation, and ultimately contributing to immune evasion by tumors, acceptance of semi-allogeneic fetuses, and survival of transplanted grafts; its surface expression can be generated through alternative splicing of seven reported isoforms (HLA-G1 to -G7), with HLA-G1 being the full-length membrane-bound form that can also be proteolytically cleaved to generate soluble HLA-G5, while the other isoforms are either soluble or anchored via glycolipid, and its expression is tightly regulated by genetically polymorphic 5′-upstream regulatory elements, hypoxia, cytokines (IL-10, IFN-β, GM-CSF), and microRNAs, making HLA-G a key diagnostic, prognostic and therapeutic target in pregnancy complications, transplantation, autoimmunity and cancer.

  • 免疫印迹

    • WB result of HLA-G Recombinant Rabbit mAb
      Primary antibody: HLA-G Recombinant Rabbit mAb at 1/10000 dilution
      Lane 1: LNCaP whole cell lysate 20 µg
      Lane 2: SK-OV-3 whole cell lysate 20 µg
      Lane 3: U937 whole cell lysate 20 µg
      Lane 4: Jurkat whole cell lysate 20 µg
      Lane 5: JEG-3 whole cell lysate 20 µg
      Negative control: LNCaP whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 38 kDa
      Observed MW: 38, 40 kDa

FAQs

斯达特公司的抗体,可以回收利用几次?

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定

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