Histone H3.3 Recombinant Rabbit mAb (S-3883)

H3.3A,H3F3,H3F3A,H3-3A

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6479

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3.3
分子别名

H3.3A; H3F3; H3F3A; H3-3A
细胞定位

Nucleus
Accession

P84243
克隆号

S-3883
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt, Mk
阳性样本

Ramos, Raji, HeLa, PC-3, NIH/3T3, mouse colon, rat stomach, COS-7
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt, Mk
IHC-P 1:2000 Hu, Ms, Rt
ICC 1:500 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu, Ms, Rt
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

背景介绍

Histone H3.3 is a replication-independent variant of the canonical histone H3 that can be deposited into nucleosomes throughout the cell cycle by dedicated chaperones such as HIRA, DAXX/ATRX, and DEK; it plays pivotal roles in gene activation, epigenetic memory, and chromatin remodeling because its distinct amino-acid sequence—especially residues that differ from H3.1/H3.2 like Ser31, Ala87, and Val89—alters nucleosome stability and creates preferential binding surfaces for transcription factors and histone-modifying enzymes, thereby influencing processes ranging from stem-cell pluripotency and development to neuronal activity and oncogenesis when its incorporation or mutations (e.g., the oncohistone Gly34Arg or Lys27Met) become dysregulated.

免疫印迹
- WB result of Histone H3.3 Recombinant Rabbit mAb
  Primary antibody: Histone H3.3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Ramos whole cell lysate 20 µg
  Lane 2: Raji whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
- WB result of Histone H3.3 Recombinant Rabbit mAb
  Primary antibody: Histone H3.3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse colon lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
- WB result of Histone H3.3 Recombinant Rabbit mAb
  Primary antibody: Histone H3.3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat stomach lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
- WB result of Histone H3.3 Recombinant Rabbit mAb
  Primary antibody: Histone H3.3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3.3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3.3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Histone H3.3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat stomach. Anti-Histone H3.3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- Histone H3.3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3.3 Recombinant Rabbit mAb (S-3883) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3.3 Recombinant
  Rabbit mAb (S-3883)-immunoprecipitated
  sample.
组织表达图谱
- Expression of Histone H3.3 in tumor tissue.
- Expression of Histone H3.3 in human tissue.
- Expression of Histone H3.3 in mouse & rat tissue.