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Rabbit抗原名称
Hamartin/TSC1分子别名
Tuberous sclerosis 1 protein; KIAA0243; TSC免疫原
Synthetic Peptide细胞定位
Cytoplasm, LysosomeAccession
Q92574克隆号
S-2725-166抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HeLa, 293T, IMR-32, mouse skeletal muscle, mouse heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:250 Hu
Hamartin/TSC1 is a 1,164-amino-acid tumor-suppressor scaffold that, together with tuberin (TSC2), forms the TSC complex that integrates growth-factor, energy and stress signals to dock at the lysosome and switch Rheb-GTP to Rheb-GDP, thereby allosterically locking mTORC1 in its inactive state; TSC1 stabilizes TSC2 via its N-terminal coiled-coil and C-terminal TBC-like domains, and pathogenic TSC1 mutations that disrupt this interaction unleash constitutive mTORC1 signaling, causing tuberous sclerosis complex with multi-organ hamartomas, epilepsy and autism spectrum phenotypes, while systemic or brain-specific TSC1 deletion in mice phenocopies these lesions and makes the protein a key pharmacodynamic node for rapamycin-class drugs.
免疫印迹
WB result of Hamartin/TSC1 Recombinant Rabbit mAb
Primary antibody: Hamartin/TSC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: IMR-32 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 130 kDa
Observed MW: 140 kDaWB result of Hamartin/TSC1 Recombinant Rabbit mAb
Primary antibody: Hamartin/TSC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 130 kDa
Observed MW: 140 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Hamartin/TSC1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Hamatrin/TSC1 in tumor tissue.
Expression of Hamatrin/TSC1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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