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eEF2K Rabbit Polyclonal Antibody

Eukaryotic elongation factor 2 kinase,eEF-2 kinase,eEF-2K,Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase,EEF2K

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6474

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

eEF2K
分子别名

Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; EEF2K
免疫原

Recombinant Protein
细胞定位

Synapse, Cytoplasm
Accession

O00418
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HEK-293, 293T, HeLa, K562, PC-3, COS-7
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Mk
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Mk
ICC	1:500	Hu

背景介绍

eEF2K (eukaryotic elongation factor 2 kinase) is a highly conserved Ca²⁺/calmodulin-dependent serine/threonine kinase that acts as a critical metabolic checkpoint by phosphorylating and inactivating eukaryotic elongation factor 2 (eEF2) at Thr56, thereby shutting down the energy-intensive translocation step of ribosomal translation elongation to conserve ATP during nutrient starvation, hypoxia, or oxidative stress; beyond this canonical translational control, eEF2K also exerts non-canonical, kinase- or eEF2-independent functions—modulating transcription factors such as p53, promoting DNA-damage repair, maintaining mitochondrial quality, and enhancing tumor cell survival in nutrient-poor microenvironments—while its own activity is tightly regulated by multiple upstream signaling pathways including mTORC1-mediated inhibition and AMPK- or TRPM7-mediated activation, making it a potential biomarker and therapeutic target in cancer and metabolic diseases.

免疫印迹
- WB result of eEF2K Rabbit pAb
  Primary antibody: eEF2K Rabbit pAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: K562 whole cell lysate 20 µg
  Lane 5: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 100 kDa
- WB result of eEF2K Rabbit pAb
  Primary antibody: eEF2K Rabbit pAb at 1/1000 dilution
  Lane 1: COS-7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 100 kDa
免疫细胞化学
- ICC shows positive staining in HeLa cells. Anti- eEF2K antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).