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Rabbit抗原名称
CALHM2分子别名
Calcium homeostasis modulator protein 2; Protein FAM26B; FAM26B免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9HA72克隆号
S-2671-146抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Mk阳性样本
U-2 OS, HeLa, U-87 MG, NIH/3T3, COS-7预测反应种属
(反应种属缩写表)Bv纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Mk IHC-P 1:50 Hu ICC 1:50 Hu
CALHM2 (Calcium Homeostasis Modulator 2) is a 323-amino-acid, four-pass transmembrane protein that oligomerizes into an undecameric, voltage-gated channel which—in addition to its originally described roles in taste-bud ATP release and neuronal excitability—has recently been shown to reside in the inner mitochondrial membrane where it functions as a Ca²⁺-inhibited import channel that translocates the ECHA subunit of the mitochondrial trifunctional protein into the matrix to enable fatty-acid β-oxidation, cristae integrity and normal respiratory metabolism, thereby coupling ion-channel gating to cellular energy supply and implicating CALHM2 in metabolic and neuropsychiatric disorders.
免疫印迹
WB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDaWB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDaWB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
This blot was developed with high sensitivity substrate
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in U2-OS cells. Anti-CALHM2 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of CALHM2 in tumor tissue.
Expression of CALHM2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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