EpCAM Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-078-128)
Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein (EGP),Epithelial glcoprotein 314 (EGP314,hEGP314),GA733-2,M1S2,M4S1,MIC18,TACSTD1,TROP1,Ber-EP4,CD326
分享产品介绍 评论(0)
宿主来源
Rabbit分子别名
Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein (EGP), Epithelial glcoprotein 314 (EGP314; hEGP314), GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, Ber-EP4, CD326免疫原
Synthetic Peptide细胞定位
Cell junction, Lateral cell membraneAccession
P16422克隆号
S-078-128抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICC ?
IF ?
稀释度
应用 稀释度 推荐种属 ICC 1:2000 Hu IF 1:2000 Hu, Ms, Rt
Epithelial cell adhesion molecule (EpCAM), also known as CD326 among other names, is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.
免疫细胞化学
ICC shows positive staining in HT-29 cells. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows negative staining in Jurkat cells. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
免疫荧光
IF shows positive staining in paraffin-embedded human colon cancer. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human endometrial cancer. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse colon. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat colon. Anti-EpCAM (Alexa Fluor® 488 Conjugate) antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
评论(0)