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Rabbit抗原名称
TACC3分子别名
Transforming acidic coiled-coil-containing protein 3; ERIC-1; ERIC1免疫原
Synthetic Peptide细胞定位
Cytoplasm, CytoskeletonAccession
Q9Y6A5克隆号
S-3048-50抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
RPMI-8226, HeLa, Raji, Ramos, mouse testis, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
TACC3 (Transforming Acidic Coiled-Coil Containing Protein 3) is a centrosomal and microtubule-associated protein essential for bipolar spindle assembly and chromosome segregation during mitosis and meiosis. It stabilizes microtubules by forming a complex with ch-TOG (CKAP5) and clathrin, promoting kinetochore-microtubule attachments and preventing spindle multipolarity. TACC3 is frequently overexpressed or involved in oncogenic fusions like FGFR3-TACC3 in various cancers, including bladder, pancreatic, and colorectal cancers, where it drives proliferation, immune evasion, and chemoresistance. Its overexpression disrupts DNA damage response pathways and sensitizes cells to PARP inhibitors, while its depletion induces mitotic catastrophe and cell cycle arrest, making it a promising therapeutic target in oncology.
免疫印迹
WB result of TACC3 Recombinant Rabbit mAb
Primary antibody: TACC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RPMI-8226 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: Ramos whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 120 kDaWB result of TACC3 Recombinant Rabbit mAb
Primary antibody: TACC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 110 kDaWB result of TACC3 Recombinant Rabbit mAb
Primary antibody: TACC3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-TACC3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Ramos cells. Anti-TACC3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of TACC3 in tumor tissue.
Expression of TACC3 in human tissue.
Expression of TACC3 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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