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Rabbit抗原名称
RBPMS分子别名
RNA-binding protein with multiple splicing; RBP-MS; Heart and RRM expressed sequence (Hermes); HERMES免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
Q93062克隆号
S-2387-235抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt, Mk阳性样本
HepG2, A549, A431, HeLa, mouse heart, rat heart, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:200 Hu, Ms, Rt ICC 1:500 Hu IF 1:500 Ms
RBPMS (RNA-binding protein with multiple splicing) is an RNA-recognition-motif protein that binds tandem CAC motifs in pre-mRNAs to orchestrate tissue-specific alternative splicing, mRNA stability and translational control; in the heart it regulates inclusion of sarcomeric exons (e.g., Ttn exon 47, Pdlim5, Nexn) essential for post-natal contractility, so its loss causes dilated cardiomyopathy and heart failure, while in retina and neurons it serves as a selective marker of retinal ganglion cells and modulates axonal integrity, and during development it governs cardiomyocyte cytokinesis, preventing premature binucleation and non-compaction cardiomyopathy, thereby exerting stage- and cell-type-specific control over RNA processing critical for vision, neural function and cardiac performance.
免疫印迹
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Low expression control: U937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-30 kDaWB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Low expression control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-26 kDaWB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat heart lysate 20 µg
Low expression control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-30 kDaWB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-26 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A549 cells. Anti-RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
免疫荧光
IF shows positive staining in paraffin-embedded mouse retina. Anti-RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of RBPMS in tumor tissue.
Expression of RBPMS in human tissue.
Expression of RBPMS in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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