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Rabbit抗原名称
Phospho-eIF4E (Ser209)分子别名
Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4EL1; EIF4F免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P06730克隆号
S-2652-195抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Hu, Ms, Rt, Mk预测反应种属
(反应种属缩写表)Bv, Xe纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt, Mk IHC-P 1:500 Hu, Ms, Rt
Phospho-eIF4E (Ser209) refers to the activated form of the eukaryotic translation initiation factor 4E following the phosphorylation of its serine residue at position 209. This event is a critical regulatory step in controlling the initiation of protein synthesis. eIF4E itself is the key initiation factor that recognizes and binds to the 5' cap structure of mRNA, serving as the core component of the eIF4F complex responsible for recruiting the ribosome to the mRNA. When its Ser209 site is phosphorylated by the specific kinases MNK1 and MNK2, the affinity of eIF4E for the cap structure is significantly enhanced, and its binding to eIF4G is promoted. This process preferentially accelerates the translation efficiency of a specific class of mRNAs, which typically possess highly complex 5' untranslated regions and encode proteins crucial for cell proliferation, survival, migration, and angiogenesis (such as c-Myc, Cyclin D1, and VEGF). This phosphorylation event is a key downstream effect of the Ras/MAPK signaling pathway. Notably, in cancer, the level of Phospho-eIF4E (Ser209) is often abnormally elevated, leading to increased synthesis of oncogenic proteins that drive tumor initiation, progression, invasion, and resistance to chemotherapy. Consequently, it is regarded as a highly promising therapeutic target and prognostic biomarker in oncology.
免疫印迹
WB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDaWB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDaWB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDaWB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
斑点杂交
Dot blot result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Lane 1: eIF4E (S209) phospho peptide
Lane 2: eIF4E unmodified peptide
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human prostate. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Phospho-eIF4E (Ser209) in tumor tissue.
Expression of Phospho-eIF4E (Ser209) in human tissue.
Expression of Phospho-eIF4E (Ser209) in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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