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Mouse抗原名称
p38 delta/MAPK13 + p38 alpha/MAPK14分子别名
Mitogen-activated protein kinase; MAP kinase; MAPK细胞定位
Cytoplasm, NucleusAccession
Q16539, O15264克隆号
S-3860抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat, A431, NIH/3T3预测反应种属
(反应种属缩写表)BV, Dg, Hm, AfGrMk纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms
MAPK13 and MAPK14, commonly known as p38δ and p38α respectively, are critical members of the p38 mitogen-activated protein kinase (MAPK) signaling pathway. Functioning as essential intracellular signaling molecules, they are primarily activated in response to diverse stress stimuli, such as inflammatory cytokines, environmental stress, and oxidative stress. Their activation depends on the dual phosphorylation of specific threonine and tyrosine residues, which in turn regulates the activity of numerous downstream transcription factors and proteins. MAPK14 (p38α) is the most extensively studied and ubiquitously expressed isoform, playing a central role in processes like inflammatory responses, apoptosis, cell differentiation, and cell cycle regulation, making it a significant drug target for inflammatory and autoimmune diseases. In contrast, MAPK13 (p38δ) exhibits a more tissue-specific expression pattern, predominantly found in glandular tissues (e.g., salivary glands, pancreas), skin, and lungs, where it fulfills distinct roles in tissue-specific inflammation, skin pathophysiology, and the regulation of insulin secretion. Although they share upstream activators and some downstream substrates, MAPK13 and MAPK14 exhibit both overlapping and complementary physiological functions, collectively forming a core network for the precise cellular regulation of stress responses.
免疫印迹
WB result of p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb
Primary antibody: p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 39 kDaWB result of p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb
Primary antibody: p38 delta/MAPK13 + p38 alpha/MAPK14 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 39 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human esophagus. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A431 cells. Anti- p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- p38 delta/MAPK13 + p38 alpha/MAPK14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of p38 delta/MAPK13 + p38 alpha/MAPK14 in tumor tissue.
Expression of p38 delta/MAPK13 + p38 alpha/MAPK14 in human tissue.
Expression of p38 delta/MAPK13 + p38 alpha/MAPK14 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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