Olfm4 Recombinant Rabbit mAb (S-2748-67)

Olfactomedin-4,OLM4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6463

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Olfm4
分子别名

Olfactomedin-4; OLM4
免疫原

Synthetic Peptide
细胞定位

Secreted, Mitochondrion
Accession

Q3UZZ4
克隆号

S-2748-67
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Ms, Rt
阳性样本

mouse spleen, rat spleen
纯化方式

Protein A
浓度

2 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB

IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IHC-P 1:2000 Ms, Rt
IF 1:2000 Ms

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:2000	Ms, Rt
IF	1:2000	Ms

背景介绍

Olfm4 (olfactomedin-4) is a secreted glycoprotein highly expressed in intestinal stem cells, bone marrow neutrophils and prostate epithelium that acts as a context-dependent modulator of cell adhesion, innate immunity and apoptosis by binding cadherins and lectins, potentiating Wnt/Notch signaling to promote epithelial regeneration, and interacting with GRIM-19 to suppress mitochondrial apoptosis, while its frequent up-regulation in gastric, colorectal and other cancers fuels tumor growth, metastasis and chemoresistance and makes it a valuable serum biomarker.

免疫印迹
- WB result of Olfm4 Recombinant Rabbit mAb
  Primary antibody: Olfm4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse colon lysate 20 µg
  Lane 2: mouse spleen lysate 20 µg
  Negative control: mouse colon lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 100 kDa
- WB result of Olfm4 Recombinant Rabbit mAb
  Primary antibody: Olfm4 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat colon lysate 20 µg
  Lane 2: rat spleen lysate 20 µg
  Negative control: rat colon lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 57 kDa
  Observed MW: 100 kDa
  This blot was developed with high sensitivity substrate
免疫沉淀
免疫组化
- IHC shows positive staining in paraffin-embedded mouse small intestine. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse lung. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse colon. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat lung. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat spleen. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat colon. Anti-Olfm4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded mouse small intestine. Anti- Olfm4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse spleen. Anti- Olfm4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows positive staining in paraffin-embedded mouse lung. Anti- Olfm4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
- IF shows negative staining in paraffin-embedded mouse colon. Anti- Olfm4 antibody was used at 1/2000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
- Expression of Olfm4 in mouse & rat tissue.