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Rabbit抗原名称
MYSM1分子别名
Deubiquitinase MYSM1; 2A-DUB; Myb-like; SWIRM and MPN domain-containing protein 1; KIAA1915免疫原
Recombinant Protein细胞定位
Nucleus, CytoplasmAccession
Q5VVJ2克隆号
S-2431-114抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Mk阳性样本
A431, HeLa, Jurkat, COS-7纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Mk
MYSM1 (Myb-like, SWIRM and MPN domain-containing protein 1) is a metalloprotease deubiquitinase that removes monoubiquitin from histone H2A at K119, functioning as a transcriptional coactivator by counteracting Polycomb-mediated gene silencing, and also cleaves K63-linked polyubiquitin chains from innate immune signaling molecules such as TRAF3, TRAF6, RIP2 and STING to dampen TLR, NOD2 and cGAS-STING pathways; through these dual nuclear and cytosolic activities MYSM1 regulates hematopoiesis, lymphocyte development, stem cell quiescence, inflammatory responses and tumor suppression, and its germline mutations cause a rare bone marrow failure syndrome characterized by leukopenia, anemia and multi-organ developmental defects.
免疫印迹
WB result of MYSM1 Recombinant Rabbit mAb
Primary antibody: MYSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 75, 110 kDaWB result of MYSM1 Recombinant Rabbit mAb
Primary antibody: MYSM1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 80, 120 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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