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JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb (S-3593)

Mitogen-activated protein kinase 8,MAP kinase 8,MAPK 8,JNK-46,Stress-activated protein kinase 1c (SAPK1c),Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1,PRKM8,SAPK1,SAPK1C,MAPK8,Mitogen-activated protein kinase 9,MAP kinase 9,MAPK 9,JNK-55,Stress-activated protein kinase 1a (SAPK1a),Stress-activated protein kinase JNK2,c-Jun N-terminal kinase 2,MAPK9,PRKM9,SAPK1A,Mitogen-activated protein kinase 10,MAP kinase 10,MAPK 10,MAP kinase p49 3F12,Stress-activated protein kinase 1b (SAPK1b),Stress-activated protein kinase JNK3,c-Jun N-terminal kinase 3,MAPK10,JNK3A,PRKM10,SAPK1B

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6461
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)

  • 分子别名

    Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; PRKM8; SAPK1; SAPK1C; MAPK8; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a (SAPK1a); Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK9; PRKM9; SAPK1A; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b (SAPK1b); Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAPK10; JNK3A; PRKM10; SAPK1B

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    P45983, P45984, P53779

  • 克隆号

    S-3593

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    IHC-P 1:2000 Hu, Ms, Rt
    ICC 1:100 Ms
背景介绍

  • JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) refers to the activated state of the three main isoforms of the c-Jun N-terminal Kinase family—JNK1, JNK2, and JNK3—following the phosphorylation of key residues within their activation loops. Specifically, the phosphorylation of threonine 183 (T183) in JNK1 and JNK2, and threonine 221 (T221) in JNK3, in conjunction with the phosphorylation of their respective tyrosine residues, is essential for the full activation of JNK. This dual phosphorylation induces a conformational change in the JNK protein, exposing its kinase active site. The JNK signaling pathway is a central hub for cellular responses to environmental stresses, such as ultraviolet radiation, oxidative stress, osmotic shock, and inflammatory cytokines. Upon activation, the phosphorylated JNK translocates into the nucleus, where it regulates critical cellular processes including proliferation, differentiation, apoptosis, and inflammatory responses by phosphorylating downstream transcription factors like c-Jun and ATF2. It is noteworthy that JNK1 and JNK2 are ubiquitously expressed across various tissues, whereas JNK3 is primarily found in the brain, heart, and testes and is closely associated with neuronal apoptosis. Consequently, detecting the phosphorylation levels of JNK at the T183/T221 sites is a key molecular indicator for measuring the activity of this pathway, and its aberrant activation is closely linked to the pathogenesis and progression of neurodegenerative diseases, cancer, insulin resistance, and various autoimmune disorders.

  • 免疫印迹

    • WB result of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated HeLa whole cell lysate 20 µg
      Lane 2: HeLa treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48, 53 kDa
      Observed MW: 40, 53 kDa
      This blot was developed with high sensitivity substrate

    • WB result of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb
      Blocking/Diluting buffer and concentration: 5% NFDM/TBST
      Primary antibody: JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
      Lane 2: NIH/3T3 treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 48, 53 kDa
      Observed MW: 40, 53 kDa
      This blot was developed with high sensitivity substrate

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human tonsil. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat spleen. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in NIH/3T3 cells treated with Anisomycin (250ng/ml 30min). Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows weak staining in NIH/3T3 cells. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows negative staining in lambda phosphatase treated NIH/3T3 cells. Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody was used at 1/100 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in tumor tissue.

    • Expression of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in human tissue.

    • Expression of JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

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