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产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体

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Histone H3 (tri methyl K9) Recombinant Rabbit mAb (S-3826)

H3K9me3

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6460
规格
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产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Histone H3 (tri methyl K9)

  • 分子别名

    H3K9me3

  • 细胞定位

    Nucleus

  • Accession

    P68431

  • 克隆号

    S-3826

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    甲基化

  • 反应种属 ?

    Hu, Ms, Rt, Mk

  • 阳性样本

    HeLa, NIH/3T, PC-12, COS-7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ChIP

    IHC-P ?

    ICC ?

    WB

    IF ?

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000-1:5000 Hu, Ms, Rt, Mk
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu, Ms
    IF 1:500 Hu
    ChIP 1:20-1:50 Hu
背景介绍

  • Histone H3 tri-methylated lysine 9 (H3K9me3) is a crucial epigenetic mark formed by the trimethylation of the ninth lysine residue on the core histone H3. It is widely recognized as a classic hallmark of constitutive heterochromatin. Its primary function is to recruit effector proteins like Heterochromatin Protein 1 (HP1), which subsequently promotes the compaction of chromatin into a dense, transcriptionally silent higher-order structure. This modification plays a key role in maintaining genomic stability by effectively repressing the aberrant expression of repetitive genomic elements, such as transposons and telomeric regions, as well as foreign genes. Furthermore, H3K9me3 is essential for the transcriptional regulation of specific genes, X-chromosome inactivation, and cell fate determination. It is important to note that aberrant distribution of H3K9me3 is closely associated with various diseases, including cancer development, neurodegenerative disorders, and the aging process.

  • 免疫印迹

    • WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

    • WB result of Histone H3 (tri methyl K9) Recombinant Rabbit mAb
      Primary antibody: Histone H3 (tri methyl K9) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: COS-7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 15 kDa
      Observed MW: 17 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat liver. Anti-Histone H3 (tri methyl K9) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded human ovarian cancer. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

    • IF shows positive staining in paraffin-embedded human breast cancer. Anti-Histone H3 (tri methyl K9) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • ChIP

    • Chromatin immunoprecipitation (ChIP) was performed on HeLa cells cross - linked
      with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
      Parallel reactions used Histone H3 (tri methyl K9) Recombinant Rabbit mAb (S-3826) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
      Post -immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
      qPCR (%input: immunoprecipitated DNA/input DNA)
      showed the enrichment of RPL30, GAPDH, MYOD1,
      AFM, SAT-α and SAT-2 in Histone H3 (tri methyl K9) Recombinant
      Rabbit mAb (S-3826)-immunoprecipitated sample.

  • 组织表达图谱

    • Expression of Histone H3 (tri methyl K9) in tumor tissue.

    • Expression of Histone H3 (tri methyl K9) in human tissue.

    • Expression of Histone H3 (tri methyl K9) in mouse & rat tissue.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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